Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein,monomeric hFc Tag

Description :
Source Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-terminal. It contains Met112-Leu260.[Accession |P27548] Molecular Weight The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data: Immobilized Mouse CD40, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 0.12g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.