Biotinylated Human CEACAM-5/CD66e Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species. Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5 is synthesized as a 180kDa, variably glycosylated molecule of which approximately 60%is carbohydrate. CEACAM-5 functions as acalcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEACAM-5 is up-regulated in a wide variety of human tumors and is a commonly used cancer marker. It promotes tumor cell migration, invasion, adhesion, and metastasis. It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis). 产品性质 Synonyms CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392 Uniprot No. Q8N4D0 Source Recombinant Biotinylated Human CEACAM-5/CD66e Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Lys35-Ala685. Molecular Weight The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100g/mLis recommended. Dissolve the lyophilized protein in distilled water.