Biotinylated Human IL-3 R alpha/CD123 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL_x005f_x001e_3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage_x005f_x001e_committed cells. The biological effects of IL_x005f_x001e_3 on the various cell types are mediated by the binding of IL_x005f_x001e_3 to specific cell surface receptor complexes. The functional high_x005f_x001e_affinity human IL_x005f_x001e_3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL_x005f_x001e_3 with low affinity. 产品性质 Synonyms CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha Uniprot No. P26951-1 Source Recombinant Biotinylated Human IL-3 R alpha/CD123 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Thr19-Arg305. Molecular Weight The protein has a predicted MW of 35.99 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100g/mLis recommended. Dissolve the lyophilized protein in distilled water.