Biotinylated Recombinant Human HGFR/c-MET Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 HGFR is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor.Within the ECD, human HGFR shares 86%-88% aa sequence identity with canine, mouse, and rat HGFR. 产品性质 Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor Uniprot No. P08581-1 Source Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu25-Thr932. Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into and chain. The protein has a predicted MW of 32.5 kDa ( chain) and 72.1 kDa ( chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data:Immobilized Anti-HGF R Antibody, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human HGFR, His Tag with the EC50of 25.7ng/ml determined by ELISA. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.