Biotinylated Recombinant Human TROP-2/TACSTD2 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. 产品性质 别名 EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 Uniprot No. P09758 Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 cells with His tag and Avi tag at the C-terminal. It contains His27-Thr274. 分子量 The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC ELISA Data:Immobilized Anti-TROP-2 Antibody, hFc Tag at 0.5g/mL(100L/well) on the plate. Dose response curve for Biotinylated Human TROP-2, His Tag with the EC50of 24.1ng/mLdetermined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.