Biotinylated Recombinant Human FGFR1 alpha(IIIc) Protein ,His-Avi Tag

Description :
FAQ COA 已发表文献 Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Additional splicing events for FGF R1-3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). Mutations in FGF R1-3 have been found in patients with birth defects involving craniosynostosis. 产品性质 别名 FGF R1a; FGFR1 alpha Uniprot No. P11362.7 Recombinant Biotinylated Human FGFR1 alpha (IIIc) Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Arg22-Glu374. 分子量 The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Anti-FGFR1 Anitibody, hFc Tag at 0.5g/mL(100L/well) on the plate. Dose response curve for Biotinylated Human FGFR1 alpha (IIIc) , His Tag with the EC50of 25.9ng/mLdetermined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.