Recombinant Human IgG4 Fc Protein

Description :
Source Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag. It contains Glu99-Gly326.[Uniprot|P01861] Molecular Weight The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data:Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 M as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.