Recombinant Human Complement Factor D/CFD (His Tag)

Description :
FAQ COA 已发表文献 Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, Factor D is also known as adipsin. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3. Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, Factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing Factor D activity. 产品性质 别名 Adipsin; C3 convertase activator; Complement Factor D; CFD; PFD; DF; ADN; Factor D; AMBP-1; EC 3.4.21; EC 3.4.21.46 Uniprot No. P00746.1 Recombinant Human Complement Factor D/CFDProtein is expressed from HEK293Cellswith His tag at the C-terminal. It contains Ile26-Ala253. 分子量 Approximately 25.5 kDa. Due to glycosylation, the protein migrates to 26-27 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human Complement Factor D, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.