Recombinant Human CD74/DHLAG (His Tag)

Description :
FAQ COA 已发表文献 CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading . Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface. CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the upregulation of angiogenic factors in endometrial stromal cells. MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation. CD74 is upregulated on nonimmune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques . It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis. 产品性质 别名 CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia-GAMMA ; II Accession NP_004346.1 Recombinant Human CD74/DHLAGProtein is expressed from HEK293Cellswith His tag at the N-terminal. It contains Gln73-Met232. 分子量 Approximately 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGE. ELISA Data:Immobilized Human CD74 at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.