Recombinant Human ANGPT2/Angiopoietin-2 (His-Avi Tag)

Description :
FAQ COA 已发表文献 Angiopoietin-2 (ANG-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Under reducing conditions, secreted monomeric ANG-2 is 65-66 kDa in size. Under nonreducing conditions, both natural and recombinant ANG-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers. ANG-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus . It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies. Both ANG-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2. ANG-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM1 and VCAM1 on EC, which facilitates the adhesion of leukocytes during inflammation. ANG-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue. Its direct interaction with Integrins promotes tumor cell invasion. ANG-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells. 产品性质 别名 AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand Uniprot No. O15123.1 Recombinant Human ANGPT2/Angiopoietin-2Protein is expressed from HEK293Cellswith His tag and Avi tag at the N-terminal. It contains Lys275-Phe496. 分子量 Approximately 28.4 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGE. ELISAData: Immobilized Human ANGPT2, His Tag at 2g/ml (100l/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.