Recombinant Human LILRA4/CD85g(His-Avi Tag)

Description :
FAQ COA 已发表文献 LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain. Alternative splicing generates an additional isoform that lacks the signal peptide and a portion of the first Ig-like domain. LILRA4 is expressed on plasmacytoid dendritic cells (pDC) but is down-regulated in response to TLR9 signaling. Antibody mediated crosslinking of LILRA4 on pDC inhibits the production of type I interferons following TLR7 or TLR9 stimulation. It also blocks the up-regulation of CCR7 but enhances the up-regulation of Integrin beta 7 on TLR7/9-stimulated pDC. LILRA4 associates with the ITAM-containing adaptor protein Fc epsilon RI gamma, and this complex binds to cell surface BST2/Tetherin which is expressed on monocytes, plasmacytoid and myeloid dendritic cells, B cells, and activated CD4+ and CD8+ T cells. This interaction inhibits the TLR-induced pDC production of type I interferons, IL-6, and TNF-alpha. 产品性质 别名 CD85g; ILT7; ILT-7; ILT7MGC129598; LILRA4; MGC129597 Uniprot No. P59901 Recombinant Human LILRA4/CD85gProtein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Glu24-Asn446. 分子量 Approximately 49.5 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.