Recombinant Human 4-1BB/TNFRSF9(hFc-Avi Tag)

Description :
FAQ COA 已发表文献 4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction costimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell. It can also enhance the activation-induced cell death of repetitively stimulated T cells. 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation. 产品性质 别名 CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA Uniprot No. Q07011 Recombinant Human 4-1BB/TNFRSF9Protein is expressed from HEK293Cellswith hFc tag and Avi tag at the C-terminal. It contains Leu24-Gln186. 分子量 Approximately 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Human 4-1BB Ligand Trimer, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.