Recombinant Human B7-H5/Gi24/VISTA (hFc Tag)

Description :
FAQ COA 已发表文献 VISTA (V-domain immunoglobulin suppressor of T cell activation) is an immune checkpoint receptor expressed primarily on the surface of hematopoietic cells. Specifically, VISTA is expressed on nave CD4+ T cells and regulatory T cells. And it is up regulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation, and their production of the proinflammatory cytokines IL 2 and IFN gamma. VISTA protein is also observed on the surface of cancer cells and attenuates the anti tumor immune response enabling tumor progression. In the autoimmune disease model EAE, VISTA limits disease progression by downregulating the overactive immune system. VISTA, also known as B7-H5, PD-1H, Platelet receptor Gi24, Dies1, and SISP1, is a 55 65 kDa transmembrane glycoprotein with homology to B7 like immune co stimulatory molecules. The mature human VISTA protein contains a 162 amino acid extracellular domain (ECD) with one V type Ig like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. 产品性质 别名 B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 Uniprot No. Q9H7M9 Recombinant Human B7-H5/Gi24/VISTAProtein is expressed from HEK293Cellswith hFc tag at the C-terminal. It contains Phe33-Ala194. 分子量 Approximately 44.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA Data:Immobilized Human B7-H5, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. SPR Data: Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human IGSF11, His Tag with an affinity constant of 14.44 M as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.