Biotinylated Recombinant Human 4-1BB/TNFRSF9 (His-Avi Tag)

Description :
FAQ COA 已发表文献 4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells. Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteinerich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. 41BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity. It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome. Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively. 产品性质 别名 CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA Uniprot No. Q07011 Recombinant Biotinylated Human 4-1BB/TNFRSF9Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Leu24-Gln186. 分子量 Approximately 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Human 4-1BB Ligand Trimer, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human 4-1BB, His Tag with the EC50 of 0.58g/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.