Biotinylated Recombinant Human PD-L1/B7-H1 (His-Avi Tag)

Description :
FAQ COA 已发表文献 PD-L1, also known as B7-H1, PDL1, is one of the ligands for PD-1 and plays a critical role in the regulation of T cell immunity. The PD-L1 protein is an approximately 65 kDa transmembrane glycoprotein belonging to the B7 family of immune regulatory molecules. Mature human PD-L1 protein consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain. Within the ECD, human PD-L1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. PD-L1 provides a molecular stop signal to the adaptive immune system helping to distinguish between self and foreign antigens. PD-L1 also plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. 产品性质 别名 CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 Uniprot No. Q9NZQ7.1 Recombinant Biotinylated Human PD-L1/B7-H1Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Phe19-Arg238. 分子量 Approximately 28.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Biotinylated Human PD-L1, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.