Recombinant Human BTLA(His-Avi Tag)

Description :
FAQ COA 已发表文献 B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands. BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells. Its expression is low in nave T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature nave cells. BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR crosslinking. 产品性质 别名 CD272; BTLA; BTLA1; FLJ16065; MGC129743 Uniprot No. Q7Z6A9.1 Recombinant Biotinylated Human BTLAProtein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Lys31-Ser150. 分子量 Approximately 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData:Immobilized Human BTLA, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. SPRData:Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.