Recombinant SARS-COV-2 Spike S1 (Omicron)(His Tag)

Description :
FAQ COA 已发表文献 SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses. The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Several emerging SARS-CoV-2 genomes have been identified including the Omicron, or B.1.1.529, variant. First identified in November 2021 in South Africa, the Omicron variant quickly became the predominant SARS-CoV-2 variant and is considered a variant of concern (VOC). The Omicron variant contains 15 mutations in RBD domain that potentially affect viral fitness and transmissibility. The majority of the mutations are involved in ACE-2 binding and Omicron binds ACE-2 with greater affinity, potentially explaining its increased transmissibility. Several of these mutations are also identified in facilitating immune escape and reducing neutralization activity to several monoclonal antibodies. 产品性质 别名 S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 Accession QHO60594.1 Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529)Protein is expressed from HEK293Cells with His tag at the C-terminal. It contains Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). 分子量 Approximately 76.38 kDa. Due to glycosylation, the protein migrates to 130-145 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human ACE2, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag with the EC50 of 0.8g/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.