Recombinant SARS-COV-2 Spike S1 Protein,His Tag

Description :
FAQ COA 已发表文献 SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S Protein of the SARS-CoV-2 virus binds Angiotensin-Converting Enzyme 2 (ACE-2), but with much higher affinity and faster binding kinetics . Polyclonal antibodies to the RBD of the SARS-CoV-2 S1 subunit have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy . There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient’s bloodstream, consistent with developed immunity after exposure to the SARS-CoV-2 virus. Lastly, it has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion. 产品性质 别名 S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 Accession QHO60594.1 Recombinant SARS-COV-2 Spike S1Protein is expressed from HEK293Cells with His tag at the C-terminal. It contains Gln14-Arg683. 分子量 Approximately 76.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized SARS-COV-2 Spike S1, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 19.4ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.