Recombinant Human SIRP alpha V2/CD172a Protein,hFc Tag

Description :
Source Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Glu31-Arg369.[Uniprot|AAH26692.1] Molecular Weight The protein has a predicted MW of 63.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human CD47 at 1g/ml (100l/Well). Dose response curve for Human SIRP alpha V2, hFc Tag with the EC50 of 82.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.