Recombinant Human CLEC12A/MICL/CLL-1 (His Tag)

Description :
FAQ COA 已发表文献 C-type lectin domain family 12 member A (CLEC12A), also known as C-type lectin-like molecule 1 (CLL1), dendritic cell-associated lectin 2 (DCAL-2), myeloid inhibitory C-type lectin-like receptor (MICL), killer cell lectin like receptor-1 (KLRL1) and CD371, is a member of the C-type lectin receptor superfamily. C-type lectins are the most diverse and prevalent lectin family in immunity. Particular interest has recently been attracted by the C-type lectin-like receptors on NK cells, which appear to regulate the activation/inhibitory balance of these cells, controlling cytotoxicity and cytokine production. CLEC12A receptor has emerged as a leukemia-associated and cancer stem cell marker in myeloid malignancies. CLEC12A is a myeloid lineage antigen that is highly expressed by AML cells and LSCs, but not expressed by normal hematopoietic stem cells (HSCs). The CLEC12A TriKE induced robust NK-cell specific proliferation, enhanced NK-cell activation, and killing of both AML cell lines and primary patient-derived AML blasts in vitro while sparing healthy HSCs. 产品性质 别名 MICL; CLL-1; CLEC12A; CLL1; DCAL2; DCAL-2; CD371; CD303; CLECSF11; CLECSF7; DLEC; HECL; PRO34150; DCAL-2 Uniprot No. Q5QGZ9.2 Recombinant Human CLEC12A/MICL/CLL-1Protein is expressed from HEK293Cells with His tag at the N-terminal. It contains His65-Ala265. 分子量 Approximately 24.6 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human CLEC12A at 0.2g (100l/Well) on the plate. Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.