Recombinant Human CEACAM-1/CD66a (His Tag)

Description :
FAQ COA 已发表文献 Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily . There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX. The cytoplasmic region shows one ITIM motif and a calmodulin binding site. 产品性质 别名 BGP; BGP1; BGPI; CEACAM1; CD66a Uniprot No. P13688.1 Recombinant Human CEACAM-1/CD66aProtein is expressed from HEK293Cells with His tag at the C-terminal. It contains Gln35-Gly428. 分子量 Approximately 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human CEACAM-1 at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.