Recombinant Human CD7 (His-Avi Tag)

Description :
FAQ COA 已发表文献 CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily. Mature human CD7 consists of a 155 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 39 aa cytoplasmic domain. Within the ECD, human CD7 shares 43% and 41% aa sequence identity with mouse and rat CD7, respectively. CD7 is expressed on T cells, NK cells, myeloid progenitor cells, and CD19+ B progenitor cells. Among CD8+ T cells, the CD7-bright population preferentially contains nave and memory cells, while more weak expressors are primarily effector cells. CD7 associates in cis with CD3 and CD45 and in trans with SECTM1. Ligation of CD7 with antibodies or SECTM1 costimulates the activation and proliferation of CD4+ and CD8+ T cells and NK cells. It also enhances integrin-mediated adhesion of these cells to Fibronectin, ICAM-1, and VCAM-1 . CD7 additionally mediates the Galectin-1 induced apoptosis of T cells and facilitates the fusion of HIV-1 with target cells. 产品性质 别名 CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40 Uniprot No. P09564 Recombinant Human CD7Protein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Ala26-Pro180. 分子量 Approximately 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA Data: Immobilized Human CD7,His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 7.9ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.