Recombinant Human SIRP alpha/CD172a Protein,hFc Tag

Description :
Source Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Glu31-Arg370.Uniprot |P78324-1 Molecular Weight The protein has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandSEC-HPLC Activity ELISA Data:Immobilized Human CD47, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA. ELISA Data: Immobilized Human SIRP alpha, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 26.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.