Recombinant Mouse BCMA/TNFRSF17 (hFc Tag)

Description :
FAQ COA 已发表文献 BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity. 产品性质 别名 CD269; TNFRSF17; BCMA; BCM; TNFRSF13A Uniprot No. O88472.1 Recombinant Mouse BCMA/TNFRSF17Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Met1-Thr49. 分子量 Approximately 31 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. SPRData: Human BAFF Trimer, His-Flag Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.