Recombinant Human SIRP alpha V5 Protein,His-Avi Tag

Description :
Source Recombinant Human SIRP alpha V5 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Glu31-Arg370(S42F).[uniprot |P78324 variant 5] Molecular Weight The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data:Immobilized Human SIRP alpha V5, His Tag at 1g/ml (100l/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 60ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.