Recombinant Human APOE/Apolipoprotein E(His Tag)

Description :
FAQ COA 已发表文献 ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages, and non-neuronal cells in the CNS. ApoE-containing particles transport triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism. Mature human ApoE is a 37kDa glycoprotein. ApoE3 is the most abundant of the three common alleles in human; ApoE2 and ApoE4 differ by single aa substitutions. Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. ApoE3 dampens the TNF-alpha induced inflammatory response in vascular endothelial cells. 产品性质 别名Synonyms Apolipoprotein E, Apo-E, APOE, apolipo E Uniprot No. P02649-1 Source Recombinant Human APOE/Apolipoprotein E Protein is expressed from HEK293Cellswith His tag at the N-terminal. It contains Lys19-His317. 分子量(Molecular Weight) Approximately 35.3 kDa.Due to glycosylation, the protein migrates to35-40kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity 1.SPRData: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 1.004 M as determined in SPR assay (Biacore T200). 2.SPRData: Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.