Recombinant Human NKp30/NCR3/CD337(hFc Tag)

Description :
FAQ COA 已发表文献 NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain. NKp30 is expressed on both resting and activated NKcells of the CD56dim, CD16+subset that account for more that 85% of NKcells found in peripheral blood and spleen. NKp30 is absent from the CD56bright, CD16-subset that constitutes the majority of NKcells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation. Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells. 产品性质 别名Synonyms CD337, LY117, NCR3, NKp30, 1C7, MALS Uniprot No. AAH52582 Source Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293Cellswith hFc tag at the C-terminal. It contains Leu19-Thr138. 分子量(Molecular Weight) Approximately 39.8kDa.Due to glycosylation, the protein migrates to 53-60kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISAData: Immobilized Human NKp30, hFc Tag at 0.5g/mL(100L/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50of 13.4ng/mLdetermined by ELISA. 2.SPRData: Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 23.10 nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.