Product Name :
Recombinant Human Necti2/CD112 (His-Avi Tag)
Description :
FAQ COA 已发表文献 Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. The 65kDa long isoform of human Nectin-2/CD112 (Nectin-2) consists of a 329amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21aa transmembrane segment, and a 157aa cytoplasmic domain. Within the ECD, human Nectin-2 shares 72%aa sequence identity with mouse Nectin-2. Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts. Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus. 产品性质 别名Synonyms CD112, MPH, Nectin2, PRR2, PVRL2, HVEB, PVRR2 Uniprot No. Q92692 Source Recombinant Human Nectin-2/CD112 Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gln32-Leu360. 分子量(Molecular Weight) Approximately 38.4kDa.Due to glycosylation, the protein migrates to 45-52kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISAData: Immobilized Human Nectin-2, His Tag at 1g/mL(100L/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50of 0.80g/mLdetermined by ELISA. 2.SPRData: Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 M as determined in SPR assay (Biacore T200). 3.SPRData: Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 M as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.
Storage :
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