Recombinant Human Necti1/PVRL1/CD111 (His-Avi Tag)

Description :
FAQ COA 已发表文献 Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110kDa type I transmembrane glycoprotein that is important in the formation of adherens junctions and synapses. The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96%aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts. Nectin-1 also recognizes CD96 on NK cells. Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia. In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence. 产品性质 别名Synonyms CD111, ED4, HIgRPVRR, HVEC, MGC142031, Nectin1, PRR, PRR1, PRR1MGC16207, PVRL1, PVRR1, SK-12, PVRL1, CLPED1, HIgR, OFC7, PVRR Uniprot No. Q15223 Source Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gln31-Thr334. 分子量(Molecular Weight) Approximately 36.9kDa.Due to glycosylation, the protein migrates to 52-70kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.SPRData: Human Nectin-4, hFc Tag captured on Protein A chip, can bind Human Nectin-1, His Tag with an affinity constant of 0.17M as determined in a SPR assay (Biacore T200). 2.SPRData: Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 M as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.