Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein,hFc Tag

Description :
Source Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp126 (CD3E)&Gln23-Ser116(CD3G).[uniprot |P07766(CD3E)&P09693(CD3G)] Molecular Weight The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human CD3E&CD3G, hFc Tag at 0.5g/ml (100l/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22ug/ml determined by ELISA. ELISA Data: Immobilized Human CD3E&CD3G, hFc Tag at 1g/ml (100l/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7ng/ml determined by ELISA Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.