Recombinant Human LILRB1/CD85j/ILT2(His-Avi Tag)

Description :
FAQ COA 已发表文献 LILRB1, also known as CD85j and ILT2, is a 110kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438amino acid (aa) extracellular domain (ECD) with 4tandem Ig-like domains, a 21aa transmembrane segment, and a 168aa cytoplasmic domain with 4inhibitory ITIM motifs. LILRB1 is expressed on the surface of Bcells and monocytes, as well as subsets of NKcells, memory/effector CD8+Tcells, gamma Tcells, and monocyte-derived dendritic cells. 产品性质 别名Synonyms ILT2, ILT-2, ILT2FLJ37515, LILRB1, LIR1, MIR7, CD85J, XXbac-BCX85G21.4 Uniprot No. Q8NHL6-1 Source Recombinant Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293Cellswith His tagand Avi tagat the C-terminal. It contains Gly24-His458. 分子量(Molecular Weight) Approximately 50kDa.Due to glycosylation, the protein migrates to 72-78kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human LILRB1, His Tag at 1g/mL(100L/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50of 15ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.