Recombinant Human FGFR2 alpha(IIIb)(hFc Tag)

Description :
FAQ COA 已发表文献 Four distinct genes encoding closely related FGF receptors, FGFR1-4. All four genes for FGFRs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Only the alpha isoform has been identified for FGFR3 and FGFR4. Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. 产品性质 别名Synonyms FGF R2a, FGFR2 alpha, KGFR, CD332, BBDS, BEK, BFR-1, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25, FLJ98662 Uniprot No. P21802-3 Source Recombinant Human FGFR2 alpha (IIIb) Protein is expressed from HEK293Cellswith hFc tag at the C-terminal. It contains Arg22-Glu378. 分子量(Molecular Weight) Approximately 66.4kDa.Due to glycosylation, the protein migrates to80-115kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human FGFR2 alpha IIIb, hFc tag at 0.5g/mL(100L/Well). Dose response curve for Biotinylated Anti-FGFR2 Ab., hFc Tag with the EC50of 10.0ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.