Recombinant Human BTLA(hFc Tag)

Description :
FAQ COA 已发表文献 B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules.The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands. BTLA is expressed on Tcells, Bcells, macrophages, dendritic cells, and NK cells. Its expression is low in nave Tcells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature navecells. BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+memory Tcells that are hyper-responsive to TCR crosslinking. 产品性质 别名Synonyms CD272, BTLA, BTLA1, FLJ16065, MGC129743 Uniprot No. Q7Z6A9-1 Source Recombinant Human BTLAProtein is expressed from HEK293Cellswith hFc tag at the C-terminal. It contains Lys31-Ser150. 分子量(Molecular Weight) Approximately 40.5kDa.Due to glycosylation, the protein migrates to60-70kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISAData: Immobilized Human HVEM, His Tag at 2g/mL(100L/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50of 19.9ng/mLdetermined by ELISA. 2.ELISAData: Immobilized Human BTLA, hFc Tag at 0.2g/mL(100L/Well) on the plate. Dose response curve for Biotinylated Anti-BTLA Antibody, hFc Tag with the EC50of 12.9ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.