Recombinant Human PVRIG(mFc Tag)

Description :
FAQ COA 已发表文献 Human PVRIG, also known as CD112 receptor (CD112R), is an approximately 34kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family . It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases. The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NKcells, but not in monocyte derived dendritic cells. PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. 产品性质 别名Synonyms C7orf15, CD112R, MGC104322, MGC138297, MGC2463, PVRIG, ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RalGDS/AF-6, RMO1 Uniprot No. Q6DKI7-1 Source Recombinant HumanPVRIGProtein is expressed from HEK293 Cells with mFc tag at the C-terminal. It containsThr41-Asp171. 分子量(Molecular Weight) Approximately 39.6kDa. Due to glycosylation, the protein migrates to50-65kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity 1.ELISAData: Immobilized Human PVRIG at 1g/mL(100L/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50of 8.0ng/mLdetermined by ELISA. 2.ELISAData: Immobilized Human PVRIG, mFc Tag at 1g/mL(100L/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50of 16.9ng/mLdetermined by ELISA. 3.SPRData: Human PVRIG, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 M as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.