Recombinant Human FGL1(His-Avi Tag)

Description :
FAQ COA 已发表文献 Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1), is a liver-specific secreted protein belonging to the fibrinogen superfamily, whose members share a fibrinogen domain at their C-termini. Human Hepassocin/FGL1 is a secreted homodimer consisting of 312aawith a 22 aa signal sequence and a 290aa mature protein. Mouse and rat Hepassocin/FGL1 share approximately 84% and 83%amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II . Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses. Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells, as it is upregulated during liver regeneration following partial hepatectomy, and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats . Its expression is regulated in HepG2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant . 产品性质 别名Synonyms FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand Uniprot No. Q08830 Source Recombinant Human FGL1 protein is expressed from HEK293 Cellswith His tag and Avi tag at the N-terminal. It contains Asp64-Asn305. 分子量(Molecular Weight) The protein has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA DataImmobilized Human FGL1, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50of 0.28g/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.