Recombinant Human B7-H7/HHLA2(His Tag)

Description :
FAQ COA 已发表文献 B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins. Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain. B7H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated Bcells but not on resting Bcells. B7-H7 binds to cell surface determinants on resting and mature T cells, Bcells, and monocytes as well as on immature and mature dendritic cells. Soluble B7-H7 inhibits the proliferation of activated CD4+and CD8+T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22. 产品性质 别名Synonyms B7H7; B7-H7; HHLA2; B7 Homolog 7 Uniprot No. Q9UM44-1 Source Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Ile23-Asn344. 分子量(Molecular Weight) The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data: Immobilized Human B7-H7, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 81ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.