Recombinant Human DNAM-1/CD226(hFc Tag)

Description :
FAQ COA 已发表文献 DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+Tcells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts. Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung. In activated, but not in resting NK, T, and mast cells, thecisassociation of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation. 产品性质 别名Synonyms CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 Uniprot No. Q15762 Source Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 Cellswith hFc tag at the C-terminal. It contains Glu19-Asn247. 分子量(Molecular Weight) The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA DataImmobilized Human CD155, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Human DNAM-1/CD226, hFc Tag with the EC50of 1.8g/ml determined by ELISA. 2.SPR DataHuman DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 M as determined in SPR assay 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.