Recombinant Human CTLA-4/CD152(His Tag)

Description :
FAQ COA 已发表文献 CTLA-4 (cytotoxic T-lymphocyteassociated protein4, designated CD152), is a type I transmembrane Tcell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41-43 kDa Within the ECD, human CTLA-4 shares 68%, 71% and 8386%aa sequence identity with mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3-5). Isoforms of 56-79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4. CD28, which is structurally related to CTLA-4, is constitutively expressed on nave T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) . In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation . 产品性质 别名Synonyms CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12 Uniprot No. P16410-1 Source Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Lys36-Asp161. 分子量(Molecular Weight) The protein has a predicted MW of 14.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA DataImmobilized Human CTLA-4, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50of 20.1ng/ml determined by ELISA. 2.ELISA DataImmobilized Human B7-1, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human CTLA-4, His Tag with the EC50of 66ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.