Recombinant Human Her2/ErbB2(hFc Tag)

Description :
FAQ COA 已发表文献 产品描述 ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a typeI membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. 产品性质 别名Synonyms CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW Uniprot No. P04626-1 Source Recombinant Human Her2/ErbB2 Protein is expressed from HEK293 Cellswith hFc tag at the C-terminal. It contains Thr23-Thr652. 分子量(Molecular Weight) The protein has a predicted MW of 96.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA DataImmobilized Human Her2, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50of 86.1ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.