Recombinant Human P53 R175H(HLA-A*02:01)(His-Avi Tag)

Description :
FAQ COA 已发表文献 p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth. 产品性质 别名Synonyms MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53 Uniprot No. A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC Source Recombinant Human P53 R175H (HLA-A*02:01) Complex Protein is expressed from HEK293 Cellswith His tag and Avi tag at the C-terminal. It contains Gly25-Thr305 (HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide. 分子量(Molecular Weight) The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.