Recombinant Human MAGE-A4(HLA-A*02:01)(His-Avi Tag)

Description :
FAQ COA 已发表文献 Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4. 产品性质 别名Synonyms HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4 Uniprot No. A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV Source Recombinant Human MAGE-A4 (HLA-A*02:01) Complex Protein is expressed from HEK293 Cellswith His tag and Avi tag at the C-terminal. It contains Gly25-Thr305 (HLA-A*02:01),Ile21-Met119,(B2M) and GVYDGREHTV peptide. 分子量(Molecular Weight) The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data: Immobilized Human MAGE-A4 (HLA-A*02:01) Complex, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50of 12.7ng/ml determined by ELISA. 内毒素Endotoxin g of the protein by the LAL method. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.