Recombinant Human Necti4 (hFc Tag)

Description :
FAQ COA 已发表文献 Nectin-4 is a 66kDa typeI transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90%amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively.Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with Nectin1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development. 产品性质 别名Synonyms EDSS1, LNIR, Nectin4, PRR4, NECTIN4, PVRL4 Uniprot No. Q96NY8 Source HEK293 Cells-derivedHuman Nectin-4 Protein, Gly32-Ser349 with hFctag at the C-terminal 分子量(Molecular Weight) Approximately 61.1kDa.Due to glycosylation, the protein migrates to 72-74kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity SPRData: Human Nectin-4, hFc Tag captured on Protein A chip, can bind Human Nectin-1,His Tag with an affinity constant of 0.17M as determined in a SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.