Recombinant Human CD27 Ligand/CD70(hFc Tag)

Description :
FAQ COA 已发表文献 CD27 ligand (CD27L), also named CD70, is a typeII transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. Human CD27L cDNA encodes a 193aa residue protein with a 17aa N-terminal cytoplasmic domain, a 21aa transmembrane domain and a 155aa C-terminal extracellular domain. Human and mouse CD27L share approximated 56%aa sequence identity. CD27L is expected to exist as non-covalent homotrimers. CD70 is constitutively expressed on medullary thymic epithelial cells (mTECs) in human and mouse and on certain unconventional APCs in the mouse intestine. CD70 is also exclusively expressed on DCs, Bcells, conventional- and regulatory Tcells and NKcells but only after their activation. CD70 expression is highly regulated by antigen through Pattern Recognition Receptors (PRRs), Tcell and Bcell antigen receptors (8), and further tuned by cytokines such as IL-1 alpha, 产品性质 别名Synonyms CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;Ki-24 antigen; TNFSF7G Uniprot No. P32970-1 Source Recombinant Human CD27 Ligand/CD70 Protein is expressed from HEK293 Cellswith hFc at the N-terminal. It contains Gln39-Pro193. 分子量(Molecular Weight) The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC. Activity ELISA DataImmobilized Human CD27, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50of 37.9ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.