Recombinant Human B7-H2/ICOSLG(hFc Tag)

Description :
FAQ COA 已发表文献 B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immuneregulatory molecules. Mature human B7-H2 consists of a 238 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain. Within the ECD, human B7-H2 shares 50% and 54% aa sequence identity with mouse and rat B7H2, respectively. Alternative splicing generates a long isoform that carries a 10 aa substitution for the C-terminal 3 residues. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation. Mouse and human B7-H2 exhibit cross-species binding to ICOS. The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (TFH) and germinal centers. In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can costimulate both human and mouse nave T cells and regulatory T cells (Treg). 产品性质 别名Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50 Uniprot No. O75144-1 Source HEK293 Cells-derivedHuman B7-H2/ICOSLGProtein, Asp19-Ser258with hFctag at the C-terminal. 分子量 (Molecular Weight) The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result. 外观 (Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA Data: Immobilized Human ICOS (C136S,C137S) , His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50of 8.9ng/ml determined by ELISA. 2.SPR Data: Human B7-H2 captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.