Recombinant Human B7-1/CD80(hFc Tag)

Description :
FAQ COA 已发表文献 B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature human B7-1 consists of a 208amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21aa transmembrane domain, and a 25 aa cytoplasmic domain. Within the ECD, human B7-1 shares 50% aa sequence identity with mouse B7-1. Alternative splicing generates a 30kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4. B7-1 is expressed on activated Bcells, activated Tcells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. 产品性质 别名Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7 Uniprot No. P33681-1 Source HEK293 Cells-derivedHuman B7-1/CD80Protein, Val35-Asn242with hFc tag at the C-terminal 分子量(Molecular Weight) The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data:Immobilized Human B7-1, hFc Tag at 0.5g/ml (100L/Well) on the plate. Dose response curve for Human CTLA-4, His Tag with the EC50of 66ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.