Recombinant Human NKG2A&CD94 Protein,hFc-Flag Tag

Description :
Source Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-terminal. It contains Arg100-Ala219(NKG2A)&Ser34-Ile179 (CD94).[uniprot |P26715-1(NKG2A)&Q13241-1(CD94)] Molecular Weight The protein has a predicted MW of 17.9 kDa (CD94)&41.1 kDa (NKG2A). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human NKG2A&CD94, hFc tag at 1g/ml (100l/Well) on Fc Antibody (5g/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Ab., hFc Tag with the EC50 of 22.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.