Recombinant Human 2B4/CD244/SLAMF4(His Tag)

Description :
FAQ COA 已发表文献 2B4, also known as CD244 and SLAMF4, is a 66kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically. Mature human 2B4 consists of a 208 aaECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs. Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%-24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma T cells, monocytes, some CD4+and CD8+T cells, and some dendritic cells. CD48 mediates 2B4+cell interactions with nearly all hematopoietic cell types, including cells of the same type. 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. 产品性质 别名Synonyms 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4 Uniprot No. Q9BZW8-2 Source HEK293 Cells-derivedHuman 2B4/CD244/SLAMF4 Protein, Cys22-Arg221with His tag at the C-terminal 分子量(Molecular Weight) Approximately 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data:Immobilized Human 2B4, His Tag at 1g/mL(100L/Well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50of 0.2g/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.