Recombinant Human M-CSF R/CSF1R/CD115(hFc Tag)

Description :
FAQ COA 已发表文献 CSF1R also known as M-CSF Receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.The human M-CSFR ECD shares 60%,64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSFR, respectively.M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. M-CSF R and integrin alphavbeta3share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis. In the brain, microglia expressing increasedM-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear. 产品性质 别名Synonyms CSF1R,CSF-1-R,CSFR,FMSFIM2,MCSFR,M-CSFR,C-FMS,FIM2,FMS,HDLS Uniprot No. P07333-1 Source HEK293 Cells-derivedHuman M-CSF R / CSF1R / CD115 Protein, Ile20-Glu512 with hFctag at the C-terminal 分子量(Molecular Weight) Approximately 81.3kDa.Due to glycosylation, the protein migrates to 100-130kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA Data: Immobilized Human M-CSF at 0.5g/mL(100L/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50of 12.4ng/mLdetermined by ELISA. 2.SPRData: Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.