Recombinant Human LAIR1/CD305(hFc Tag)

Description :
FAQ COA 已发表文献 LAIR1 is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.Mature human LAIR1 is a 266amino acid (aa) typeI transmembrane protein that includes a 144 aa extracellular domain (ECD) with one collagen-binding C2-type Ig-like domain, and a 101aa cytoplasmic domain with two ITIM motifs. Human LAIR1 ECD shares LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. 产品性质 别名Synonyms CD305,HLAIR1,LAIR1,LAIR-1 Uniprot No. Q6GTX8-1 Source HEK293 Cells-derivedHuman LAIR1 / CD305 Protein, Gln22-His163with hFctag at the C-terminal 分子量(Molecular Weight) Approximately 42.4kDa.Due to glycosylation, the protein migrates to 60-70kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data: Immobilized Human Collagen I, No Tag at 5g/mL(50L/well) on the plate. Dose response curve for Human LAIR1, hFc Tag with the EC50of 1.3g/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.