Recombinant Human PCT/Procalcitonin

Description :
FAQ COA 已发表文献 ProCalcitonin (PCT) belongs to a group of related proteins including Calcitonin gene-related peptides I and II, amylin, adrenomodulin and Calcitonin (CAPA peptide family). Mature PCT is expressed as a 116amino acid (aa) protein which is subsequently cleaved into 3parts: a 57aa pro-region, a 32aa Calcitonin peptide and a 21 aa Katacalcin peptide. PCT is a peptide precursor of the hormone Calcitonin, the latter being involved with calcium homeostasis. PCT is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. But its level is related to the severity of bacterial sepsis, it is considered to be one of the earliest and most specific markers of sepsis. Product Properties Synonyms CALC1, CALCA, Calcitonin 1, Calcitonin gene-related peptide 1, CGRP, Katacalcin, ProCalcitonin Accession P01258-1 GeneID 796 Source E.coli-derived human PCT/ProCalcitonin protein, Ala26-Asn141. Molecular Weight Approximately 12.8 kDa. AA Sequence APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >98% by SDS-PAGE and HPLC analyses. Biological Activity Testing in progress. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.